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Behavioral testing for animals subjected to the 24 h minipumps began 1 hour after pump implantation and was repeated at 2, 8, 24, 25, 26, 28, and 32 h.

Behavioral testing for animals subjected to 72 h minipump began 8 hour after pump implantation and was repeated at 10, 12, 24, 36, 48, 60, 72, 80 h.


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These channels history increased excitability and neurotransmission accompanying pathological factors including neuropathic pain.
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Control minipumps that were implanted in injured animals delivered 0. In vivo microdialysis to measure dopamine release from nucleus accumbens shell NAc 2.


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The plasma of each stimulus was not 3 s and the inter-stimulus interval was approximately 15 s.
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Louis, MO according to the brain atlas. Stainless steel dummy cannulas were inserted to keep the guide cannula free of debris. After surgery, rats were housed individually and allowed to recover for 7 days.

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The placement of the guide cannula was verified histologically post hoc. In vivo microdialysis procedures Microdialysis was done in awake, freely moving rats with sham surgery or with TNI.

A standard curve was produced from 6 serial dilutions of DA 1. The linearity of DA peaks was also validated. The integration of the DA peaks from HPLC chromatograms was performed by an experimenter blinded to the treatment groups.

The percent change of the corresponding baseline level was calculated to normalize the variations of individual rats and allow for multiple comparisons.

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Three-chambered rat-CPP apparatus San Diego Instruments, San Diego, CA with the following modifications were used: 1 end chamber of horizontal black and white striped walls, smooth floor; 2 end chamber of black walls, rough floor; and 3 middle chamber of neutral gray walls and metal rod flooring.

Female Sprague-Dawley rats 200—225 g, Harlan were implanted with intracerebroventricular cannula i.

Although we have previously observed penetration of tat-CBD 3 into the brain after an intraperitoneal injection, here we chose the i. After a recovery period 7 days, rats were put in CPP boxes for 20-minute sessions and allowed to freely explore 2 end chambers and a middle transition zone with unique environmental cues in each end chamber.

Baseline measurements were obtained for each rat on days 1 through 4. The average baseline per rat was determined across days and used to calculate the mean time spent in each chamber.

On days 6, 10, and 12, animals received an injection of vehicle saline 5 mL.

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On days 13 to 14, in a peptide-free state, the total time spent by the rat was recorded and compared with baseline values for each chamber paired t test. Preference was demonstrated if there was a significant increase in the total time spent in the conditioning chamber when compared to baseline values.

Assessment of potential CNS side effects 2.

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  • Light-Dark Box test as a measure of anxiety-associated behavior For the light-dark box test LDBT, there were two adjoined light and dark compartments boxes with a 7 cm square opening to allow mice to move between them.

    Each mouse was placed into the white compartment and the white compartment was video recorded for later analyses using ANY-maze software version 4. Angelo aubreyk 73 lycos. Dannie shermann 59 lycos.