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So far, little is known about the biology and pathogenesis of S.
However, the natural host of S. Its similarities with adverse pregnancy outcomes-related pathogens such as C.
In order to precise its natural host and mode of transmission, we characterized the intracellular growth cycle of S.
We also investigated its growth within mammalian hosts such as endometrial cells and pneumocytes in order to evaluate its potential implications in human diseases such as pneumonia and miscarriages. Finally, we further investigated its intracellular traffic and characteristics of its replicative vacuole.
Infection was performed with a 7-day-old S. Briefly, cell cultures were harvested using a cell scraper and bacteria were released from cells using glass beads.
Recovered bacteria were diluted in fresh medium and used to infect cells. A quantitative analysis of the bacterial particles produced at day 7 was performed using a specific quantitative polymerase chain reaction qPCR.
Dilution was adjusted to correspond to an inoculum of 25 or 5 bacteria per cell according to the various experiments see results. Then, infected cells were washed with phosphate-buffered saline PBS to remove non-internalized bacteria prior to the addition of fresh media.
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